[Influence of the genotype on the polytene chromosome puffing and bioelectrical properties of cellular nuclei of Drosophila melanogaster treated with ecdysone in vivo and in vitro experiments].

Activity of polytene chromosome puffs at the 0-hour prepupae stage and bioelectric properties of cellular nuclei of salivary glands were investigated under the influence of the hormone 20-OH-ecdysone in vitro in highly inbred selected lines LA (low activity) and HA (high activity) and their F1 hybrids LA x HA and Ha x LA. The inadaptive line LA differs from the line HA by the smaller size of the puffs. The hybrids exceed the best parental line by the size of the majority of the investigated puffs.

[Changes in the function of the nucleus and chromatin of human cells under the action of hormonal factors in vitro].

The influence of hormones--adrenaline, noradrenaline, hydrocortisone on the electronegativity of cell nuclei (ENN) was shown by the method of intracellular microelectrophoresis. Changes in heterochromatin state were estimated on the basis of heterochromatin granules (chromocenters) quantity. Adrenaline and noradrenaline induced the decrease in ENN, the maximum effect was revealed with hormones concentration observed in human blood during physical training.