Saponin is Essential for the Isolation of Proteins and RNA from Biological Nanoparticles.

Extracellular vesicles (EVs), biomimetics, and other biological nanoparticles (BNs) produced from human cells are gaining increasing attention in the fields of molecular diagnostics and nanomedicine for the delivery of therapeutic cargo. In particular, BNs are considered prospective delivery vehicles for different biologics, including protein and RNA therapeutics. Moreover, EVs are widely used in molecular diagnostics for early detection of disease-associated proteins and RNA.

Development, production and characterization of SARS-CoV-2 virus-like particles (Coronaviridae: ).

Introduction: The COVID-19 pandemic caused by SARS-CoV-2 has created serious health problems worldwide. The most effective way to prevent the occurrence of new epidemic outbreaks is vaccination. One of the modern and effective approaches to vaccine development is the use of virus-like particles (VLPs).

Controlling the Quality of Nanodrugs According to Their New Property-Radiothermal Emission.

Previous studies have shown that complexly shaped nanoparticles (NPs) have their intrinsic radiothermal emission in the millimeter range. This article presents a method for controlling the quality of nanodrugs-immunobiological preparations (IBPs)-based on the detection of their intrinsic radiothermal emissions. The emissivity of interferon (IFN) medicals, determined without opening the primary package, is as follows (µW/m): IFN-α2b-80 ± 9 (10 IU per package), IFN-β1a-40 ± 5 (24 × 10 IU per package), IFN-γ-30 ± 4 (10 IU per package).

[Study of the safety and immunogenicity of VLP-based vaccine for the prevention of rotavirus infection in neonatal minipig model].

Introduction: In Russia, almost half of the cases of acute intestinal infections of established etiology in 2022 are due to rotavirus infection (RVI). There is no specific treatment for rotavirus gastroenteritis. There is a need to develop modern, effective and safe vaccines to combat rotavirus infection that are not capable of multiplying (replicating) in the body of the vaccinated person.

The effectiveness of the use of selenium-containing top dressing in the cultivation of radish.

Vegetable crops of the Brassicaceae family have the ability to include the necessary trace element selenium (Se) in the composition of organic compounds such as selenoproteins, in addition, they have important properties for human health based on the content of selenium. In our work, we investigated the effect of non-root processing of vegetating radish plants on the quality of finished products. The research results showed that the selenium content in the product part of plants significantly increased with an increase in the concentration of this element in the working solution.