Tools for live-cell imaging of cytoskeletal and nuclear behavior in the unconventional yeast, .

is a ubiquitous fungus with a wide variety of morphologies and growth modes including "typical" single-budding yeast, and interestingly, larger multinucleate yeast than can make multiple buds in a single cell cycle. The study of promises to uncover novel cell biology, but currently tools are lacking to achieve this goal. Here, we describe initial components of a cell biology toolkit for , which is used to express and image fluorescent probes for nuclei as well as components of the cytoskeleton.

Effect of 5'-adenosine monophosphate-activated protein kinase agonists on insulin and glucose dynamics in experimentally induced insulin dysregulation in horses.

Background: 5'-adenosine monophosphate-activated protein kinase (AMPK) agonists, particularly resveratrol (RES), have not been extensively evaluated for their effect on insulin dysregulation (ID) in horses.

Objectives: Evaluate the effects of treatment with RES (10 mg/kg PO q12h), metformin (MET; 30 mg/kg PO q12h), and aspirin (ASP; 20 mg/kg PO q24h) on experimentally induced ID.

Animals: Thirty-three healthy, adult, light-breed horses.

Distributions of Cisco (Coregonus artedi) in the upper Great Lakes in the mid-twentieth century, when populations were in decline.

The restoration of the once abundant Cisco (Coregonus artedi) is a management interest across the Laurentian Great Lakes. To inform the restoration, we (1) described historical distributions of Cisco and (2) explored whether non-indigenous Rainbow Smelt (Osmerus mordax) and Alewife (Alosa pseudoharengus) played a role in the decline of Cisco populations across the upper Great Lakes (i.e.

Mechanism of commitment to a mating partner in .

Many cells detect and follow gradients of chemical signals to perform their functions. Yeast cells use gradients of extracellular pheromones to locate mating partners, providing a tractable model for understanding how cells decode the spatial information in gradients. To mate, yeast cells must orient polarity toward the mating partner.

Trophic transfer efficiency in the Lake Superior food web: assessing the impacts of non-native species.

Ecosystem-based management relies on understanding how perturbations influence ecosystem structure and function (e.g., invasive species, exploitation, abiotic changes).