Insight into the origin of chikungunya virus in Malaysian non-human primates via sequence analysis.

Chikungunya virus (CHIKV) is maintained in the sylvatic cycle in West Africa and is transmitted by mosquito species to monkeys. In 2006, four verified CHIKV isolates were obtained during a survey of arboviruses in monkeys () in Pahang state, Peninsular Malaysia. RNA was extracted from the CHIKV isolates and used in reverse transcription polymerase chain reactions (RT-PCR) to amplify PCR fragments for sequencing.