Subcutaneous infection caused by Dirofilaria repens imported to Slovenia.

A case of human subcutaneous dirofilariasis is presented. A complete nematode removed from the tumour in the occipital region of a 61-year-old woman was identified, on the basis of its anatomical and histological characteristics, as a nongravid adult female of the species Dirofilaria repens. The patient had probably been infected on the Canary Islands, Spain, 7 months previously.

Effect of pH on Ki-67 antigen retrieval in renal cell carcinoma.

The efficacy of Ki-67 antigen retrieval (AR) was assessed in 20 formalin fixed-paraffin embedded specimens of renal cell carcinoma using three solutions with different pH values. The AR was performed by means of microwave oven heating up to 97 degrees C for 15 minutes. Better results were obtained using solutions with pH values 8.