A CoA-Transferase and Acyl-CoA Dehydrogenase Convert 2-(Carboxymethyl)cyclohexane-1-Carboxyl-CoA During Anaerobic Naphthalene Degradation.

The CoA thioester of 2-(carboxymethyl)cyclohexane-1-carboxylic acid has been identified as a metabolite in anaerobic naphthalene degradation by the sulfate-reducing culture N47. This study identified and characterised two acyl-CoA dehydrogenases (ThnO/ThnT) and an intramolecular CoA-transferase (ThnP) encoded within the substrate-induced thn operon, which contains genes for anaerobic degradation of naphthalene. ThnP is a CoA transferase belonging to the family I (Cat 1 subgroup) that catalyses the intramolecular CoA transfer from the carboxyl group of 2-(carboxymethyl)cyclohexane-1-carboxyl-CoA to its carboxymethyl moiety, forming 2-carboxycyclohexylacetyl-CoA.

Improving the reliability of phthalate esters analysis in water samples by gas chromatography-tube plasma ionization-high-resolution mass spectrometry (GC-TPI-HRMS).

The monitoring of phthalate esters (PAEs) is challenging due to background contamination as well as the low selectivity observed when analyzing them by gas chromatography coupled to mass spectrometry (GC-MS) using electron ionization (EI). In this sense, alternative and soft ionization techniques could help to enhance the performance of the analytical determinations of PAEs in food samples. In this work, the use of a novel and soft ionization technique tube plasma ionization (TPI) has been explored to enhance the selectivity and sensitivity in the determination of PAEs in drinking water samples with GC-MS.

Ecology: Re-complexifying impoverished food webs.

Traditional conservation holds that non-native species introductions lead to the loss of ecosystem complexity and function. Yet, new research shows that they can restore complexity as well.

Development of an Ultrasonic Nebulization System for an Inverse Low Temperature Plasma Ionization Source.

An effective nebulization and evaporation of a liquid sample, like in liquid chromatography, mass spectrometry (LC-MS) couplings, is an essential requirement for the ionization of analyte molecules in the gas phase by, for example, atmospheric pressure chemical ionization (APCI) or the novel low temperature plasma (LTP)-based ion source. These LTP-based ion sources have recently gained interest in the field of atmospheric pressure ion sources, as they can cover a wide range of polarity and molecular mass. They can be used in combination with separation techniques like liquid chromatography or used as an ambient ion source.