The effect of different concentrations of N-stearoylethanolamine (NSE 18:0) on fragmentation of DNA in the tumoural and extratumour tissues of the adrenal glands in vitro was studied. In this work the following types of tissue were investigated: extratumoural tissue from patients with hormonally active tumours, benign tumour tissue (hormonally active and hormonally inactive), tissue of malignant tumours and hyperplasic tissue of the adrenal glands (Itsenko-Cushing disease). It has been established that the NSE increases the intensity of DNA fragmentation only in the tissue of hormonally inactive tumours.
View Article and Find Full Text PDFThe effect of different potassium concentrations on changes in steroidogenic acute regulatory protein (StAR) and cytochrome P-450(SCC) in human adrenal cortex tissue was studied. A rise in K+ concentration in incubation medium to 8.5 mM caused an increase in StAR and cytochrome P-450(SCC) mRNA level in adrenocorticocytes by 70 and 76%, respectively, compared with control.
View Article and Find Full Text PDFThe influence of N-stearoylethanolamine (NSE) on total 11-hydroxycorticosteroids (11-HCS) and testosterone level in the blood of male rats in normal conditions and under the action of 17beta-estradiol (400 mkg/kg of body weight during 3 days) was studied. It was shown that NSE administration per os (50 mg/kg of body weight during 7 days) to intact animals did not change the level of 11-HCS and of testosterone. The administration of NSE to estrogenized male rats decreased the elevated level of 11-HCS and normalized the amount of testosterone in blood.
View Article and Find Full Text PDFThe effect of 17beta-estradiol on the change of proapoptotic protein Bax mRNA level and DNA laddering in human adrenal cortex tissue was studied. The adding of 17beta-estradiol to incubation medium caused a decrease of the proapoptotic protein Bax mRNA level in adrenocorticocytes by 22% in comparison with control. Hormone influence decreases the DNA fragmentation by 50%.
View Article and Find Full Text PDFThe estradiol effect on the expression level of ERK1/2, JNK, p38 mitogen-activated protein kinases, c-Jun and c-Fos transcription factors in the adrenal cortex of male rats was studied. It was shown that a single corticotropin injection caused significant changes of the ERK1/2 protein kinases levels in the adrenal cortex. This index was 1.
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