[Different functional and structural properties of lactate dehydrogenase isozymes at different stages of Danio rerio ontogenesis].

We studied properties of lactate dehydrogenase isozymes expressed at different stages of Danio rerio ontogenesis. H4-LDH and a minor fraction H3M1 are expressed during embryonic development. The muscle isozyme (M4) appears after the beginning of muscle contractions in the embryo.

[Temperature causes structural and functional changes in lactate dehydrogenase from fish skeletal muscles].

Adaptation of fishes to low and high environmental temperatures gives rise to certain changes in kinetic and structural properties of LDH from skeletal muscles. The KM versus temperature plot for pyruvate of the enzyme isolated from fishes adapted to low temperatures has a minimum at low measurement temperatures, whereas adaptation to high temperatures leads to the enzyme showing a minimum at high temperatures. The LDH isolated from fishes adapted to low environmental temperatures is more stable relative to both thermal and urea-induced inactivation.