Publications by authors named "O S Henry"
Article Synopsis
- The study investigates the HIV epidemic among gay, bisexual, and other men who have sex with men (GBMSM) in Kenya, emphasizing their disproportionate rates of infection and the need for tailored public health strategies.
- Researchers collected dried blood spots from 2,450 participants in different counties and found that 18.5% were living with HIV, with only a small number successfully sequenced due to high rates of viral suppression.
- Results indicated that the majority of identified HIV phylogenetic clusters involved GBMSM who sought partners online and tested less frequently than recommended, highlighting important gaps in HIV prevention and care within the community.
Lectins are widely employed for the assessment of protein glycosylation as their carbohydrate binding specificities have been well characterized. In glycosylation assays, lectins are often conjugated with biotin tags, which interact with streptavidin to functionalize biosensing surfaces or recruit signal generating molecules, depending on the assay configuration. We here demonstrate that a high degree of biotin conjugation can limit total capture to streptavidin functionalized SPR surfaces due to multipoint binding, and can additionally bias the reported kinetic evaluations when measuring the interaction between lectins and glycoproteins by SPR.
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- Technology scale-up and transfer are essential for effective biomanufacturing processes, involving careful selection of bioreactor parameters based on key performance indicators (KPI).
- A study was conducted using CHO cells to express the SARS-CoV-2 spike protein in benchtop bioreactors, examining the effects of dissolved oxygen levels and aeration on cell growth and product output.
- Dynamic feeding strategies based on variables like viable cell density and oxygen uptake rate significantly improved cell concentration and protein yield compared to traditional bolus feeding methods.
Introduction: Exocrine pancreatic insufficiency (EPI) is caused by multiple clinical conditions such as cystic fibrosis and chronic pancreatitis (CP). Standard management of EPI includes pancreatic enzyme replacement therapy (PERT) along with consultation with a dietitian. While PERTs have been on the market for several decades, newer publications on their clinical efficacy and safety raised the need for a comprehensive review of the literature.
View Article and Find Full Text PDFVesicular stomatitis virus (VSV) has been increasingly demonstrated as a promising viral vector platform. As the interest over this modality for vaccine and gene therapy applications increases, the need for intensified processes to produce these vectors emerge. In this study, we develop fed-batch-based operations to intensify the production of a recombinant VSV-based vaccine candidate (rVSV-SARS-CoV-2) in suspension cultures of HEK293 cells.
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