Antimicrobial and Regenerative Effects of Placental Multipotent Mesenchymal Stromal Cell Secretome-Based Chitosan Gel on Infected Burns in Rats.

Background: There is a need for better strategies to promote burn wound healing and prevent infection. The aim of our study was to develop an easy-to-use placental multipotent mesenchymal stromal cell (MMSC) secretome-based chitosan hydrogel (MSC-Ch-gel) and estimate its antimicrobial and regenerative activity in -infected burn wounds in rats.

Methods: Proteomic studies of the MMSC secretome revealed proteins involved in regeneration, angiogenesis, and defence responses.

MAPK and Notch-Mediated Effects of Meso-Xanthin F199 Compounds on Proliferative Activity and Apoptosis of Human Melanocytes in Three-Dimensional Culture.

Meso-Xanthin (Meso-Xanthin F199™) is a highly active antiaging injection drug of the latest generation. The main acting compound is fucoxanthin, supplemented with several growth factors, vitamins, and hyaluronic acid. Previous examination of fucoxanthin on melanocytes showed its ability to inhibit skin pigmentation through different signaling pathways focused on suppression of melanogenic-stimulating receptors.

Low level of O2 inhibits commitment of cultured mesenchymal stromal precursor cells from the adipose tissue in response to osteogenic stimuli.

Mesenchymal stromal precursor cells from human lipoaspirate (lMSC) cultured at 5% O(2) formed 50% less mineralized matrix in response to osteogenic induction than cells cultured under standard conditions (20% O(2)). After lMSC percultured at 5% O(2) were transferred to normoxic conditions (20% O(2)), they produced the same amount of matrix as lMSC permanently cultured at 20% O(2). Hence, hypoxia inhibited the commitment of lMSC under the effect of osteogenic stimuli, which can be important in reparative and regenerative medicine.

Effects of hypoxic gas mixtures on viability, expression of adhesion molecules, migration, and synthesis of interleukins by cultured human endothelial cells.

The effects of short-term (3 h) and long-term (18 h) repeated hypoxic exposure (5% O(2)) on viability of endothelial cells, expression of adhesion molecules, and secretion of IL-6 and vascular endothelial growth factor were studied. The resultant number of apoptotic and necrotic cells indicates that cultured endothelium well tolerates repeated changes in partial oxygen pressure in the medium, despite the stress reaction manifesting in enhanced secretion of IL-6. Changes in number of cells carrying ICAM-1 on their surface and activation of the synthesis of vascular endothelial growth factor during long-term exposure attest to activation of angiogenesis and inhibition of apoptosis.