[STRUCTURAL AND SPATIAL ORGANIZATION OF MICROGLIA IN THE MOLECULAR LAYER OF RABBIT CEREBELLAR CORTEX].

Using the methods of immunocytochemistry and confocal laser microscopy, structural organization and spatial distribution of microgliocytes in the molecular layer of the cerebellar cortex were studied in 5 adult male rabbits. Reaction to microglial cell marker Iba-1 was highly specific, while until recently their selective detection was impossible in rabbits. In the molecular layer of the cerebellar cortex, two patterns of microgliocyte organization were observed.

[INTRANUCLEAR ACCUMULATION OF Iba-1 PROTEIN IN MICROGLIOCYTES OF THE HUMAN BRAIN].

Iba-1 protein which is a recognized marker of the microglial cells, was previously detected by the authors in the nucleus of microgliocytes. The present study was aimed to define more exactly these data using the methods of immunohistochemistry and confocal laser microscopy. The study was performed on the fragments of the human brain (n=18, age 25–78 years).

[Simultaneous demonstration of glutamate decarboxylase and synaptophysin in paraffin sections of rat cerebellum].

The article presents highly reproducible and inexpensive protocol for simultaneous demonstration of glutamate decarboxylase (GAD67), the key enzyme of gamma-aminobutyric acid (GABA) synthesis and synaptophysin (SYP), a marker protein of synaptic vesicles using confocal laser microscopy. In the cerebellar cortex, GAD labels Purkinje cells and pinceaux in their basal parts and is unevenly distributed in the neuropil of molecular and granular layers. SYP clearly marks the contours of large dendrites of Purkinje cells in molecular layer, while in the granular layers it labels parts of cerebellar glomeruli--the terminals of the mossy fibers.

[Nestin expression in ependymal cells of lateral ventricles of the rat brain in aging].

It is known that during development of the brain, with the progress of ependendymocyte differentiation from radial gliocytes, the synthesis of nestin is stopped. However, it was shown that in the ependyma of the lateral brain ventricles nestin synthesis was resumed in response to ischemic injury. The aim of the present study was to test the hypothesis of possible re-expression of nestin in the ependyma in aging.