[Effects of decarboxylated L-arginine on morphofunctional characteristics of the erythron in experimental diabetes mellitus in rats].

This paper reports the results of a study of the impact of the agmatine treatment on erythrocyte resistance to acid hemolytic, reticulocyte count and reticulocyte production index, erythrocyte surface architectonics in streptozotocin-induced diabetic rats. Our results indicate that treatment of diabetic rats with agmatine causes a suppression of erythropoiesis and increases the resistance of erythrocytes against HCl-induced hemolysis. It was shown a 10% increase in the number of young red blood cells and 35% reduction in the number of structurally transformed erythrocytes that are capable of restoring normal biconcave disc shape under physiological condition.

[The influence of low-intensity laser irradiation on the loach embryo cells].

The ultrastructural organization of loach embryo cells (Misgurnus fossilis L) at the stage of the first and the tenth embryo divisions was investigated in the control and under the influence of low intensity helium-neon laser irradiation of 5 min exposure. The effect of laser irradiation led to ultrastructural changes in cell organelles, increasing the number and size of mitochondria, and as a result their shape changes. Under the influence of laser irradiation, the activation of cellular digestion processes took place, the number of vacuoles and lysosomes increased.

[Quantitative changes of main components of erythrocyte membranes which define architectonics of cells under pttg gene knockout].

A pttg gene knockout affects the functional state of erythron in mice which could be associated with structural changes in the structure of erythrocyte membranes. The pttg gene knockout causes a significant modification of fatty acids composition of erythrocyte membrane lipids by reducing the content of palmitic acid and increasing of polyunsaturated fatty acids amount by 18%. Analyzing the erythrocyte surface architectonics of mice under pttg gene knockout, it was found that on the background of reduction of the functionally complete biconcave discs population one could observe an increase of the number of transformed cells at different degeneration stages.

[Ultrastructural changes in cells of mycobacteria affected by flurenizid derivatives].

In cells of micobacteria of all investigated samples the following ultrastructural changes were observed: disorganization of a nucleoid and citoplasm, formation of the citoplasmic vacuoles and endocellular lipide-like inclusions, and also change of the cells form into spheroid and formeless mass, disappearance of periplasmatic space, occurrence of cells of small-size and short incompletely divided cells in the samples. They were observed more often after 72 hours of exposition of the cultures with antibacterial substances, than after 24 hours of exposition. The highest concentration of these substances being used, the ultrastructural changes were more essential.

[The influence of potassium 4-toluenethiosulfonate on membrane potential and ATPase activity of plasmatic membranes of loach embryos].

The influence of novel biologically active substance potassium 4-toluenethiosulfonate in concentration of 4 x 10(-5) M on the changes of electrophysiological parameters of embryonic cells in early development of fish (Misgurnus fossilis L.) was investigated including the changes of membrane potential (TMP) and enzyme activity of plasmatic membranes of loach embryos during the period of synchronous division of blastomers in the early period of development. The evaluation of influence of these matters was studied and aperiodic changes of the level of TMP were shown.