[Nonuniform distribution and contribution of the P- and P/Q-type calcium channels to short-term inhibitory synaptic transmission in cultured hippocampal neurons].

In the present study, we investigated the sensitivity of GABAergic short-term plasticity to the selective P- and P/Q-type calcium channels blocker omega-agatoxin-IVA. To block the P-type channels we used 30 nM of this toxin and 200 nM of the toxin was used to block the P/Q channel types. The evoked inhibitory postsynaptic currents (eIPSC) were studied using patch-clamp technique in whole-cell configuration in postsynaptic neuron and local extracellular stimulation of single presynaptic axon by rectangular pulse.

[The role of N-type high-voltage-activated calcium channels in plasticity regulation of inhibitory synaptic transmission in cultured hippocampal neurons].

Now it is clear that N-type Ca2+ channels contribute to synaptic transmission at many of CNS synapses. However, it is not known whether presynaptic N-type Ca2+ channels contribute to short-term synaptic plasticity (STP) mediated by GABA release at inhibitory synapses of cultured hippocampal neurons. We studied the sensitivity of GABAergic paired pulse depression (PPD) as a common form of STP to selective N-type high-voltage-activated Ca2+ channels blocker omega-conotoxin (omegaCgTx).