Virus isolation from saliva and salivary glands of cattle naturally infected with paralytic rabies.

The infectivity of saliva, salivary and mammary glands, muscle, lung, kidney and liver of 87 cattle infected with paralytic rabies (positive viral isolation from brains) was studied. Fifty percent dilutions of saliva and tissue samples were inoculated intracerebrally into 10- to 15-day-old mice. Viral isolation in mice was confirmed by direct rabies fluorescent-antibody test and the antigenic variant of the isolates characterized by monoclonal antibodies.

Monoclonal antibody characterization of rabies virus strains isolated in the River Plate Basin.

In this study, 91 strains isolated in the River Plate Basin, South America, were examined from the epidemiological standpoint and with monoclonal antibodies (MAbs) to the nucleocapsid of rabies virus. Such strains reacted to MAbs in accordance with nine different patterns (antigenic variants). Rabies virus was isolated from 49 cattle, 21 dogs, 11 non-haematophagous bats, four vampire bats, two foxes, two horses, one buffalo, and one human.