Publications by authors named "O Ohara"
In hypoxic pulmonary hypertension (PH), pulmonary vascular remodeling is characterized by the emergence of activated adventitial fibroblasts, leading to medial smooth muscle hyperplasia. Previous studies have suggested that CD26/dipeptidyl peptidase-4 (DPP4) plays a crucial role in the pathobiological processes in lung diseases. However, its role in pulmonary fibroblasts in hypoxic PH remains unknown.
View Article and Find Full Text PDFAntibodies are powerful tools for the therapy and diagnosis of various diseases. In addition to conventional hybridoma-based screening, recombinant antibody-based screening has become a common choice; however, its application is hampered by two factors: (1) screening starts after Ig gene cloning and recombinant antibody production only, and (2) the antibody is composed of paired chains, heavy and light, commonly expressed by two independent expression vectors. Here, we introduce a method for the rapid screening of recombinant monoclonal antibodies by establishing a Golden Gate-based dual-expression vector and in-vivo expression of membrane-bound antibodies.
View Article and Find Full Text PDFPurpose: Newborn screening using dried blood spot (DBS) samples for the targeted measurement of metabolites and nucleic acids has made a substantial contribution to public healthcare by facilitating the detection of neonates with genetic disorders. Here, we investigated the applicability of non-targeted quantitative proteomics analysis to newborn screening for inborn errors of immunity (IEIs).
Methods: DBS samples from 40 healthy newborns and eight healthy adults were subjected to non-targeted proteomics analysis using liquid chromatography-mass spectrometry after removal of the hydrophilic fraction.
Article Synopsis
- The study aimed to collect real-world data on VEXAS syndrome, focusing on disease activity, treatments, remission rates, and adverse events over a prospective period.
- Researchers enrolled suspected patients in Japan, utilizing a new disease activity measure (VEXASCAF) and recorded various health metrics, including UBA1 gene variant analysis.
- Out of 55 patients, 30 were found to have pathogenic UBA1 variants, with limited remission reported, frequent adverse events including deaths and infections, and a need for high-dose glucocorticoids to manage symptoms.
Article Synopsis
- - The study examined bleeding symptoms, platelet counts, and GPIb expression in 32 patients with 22q11.2 deletion syndrome (22q11.2DS), which affects platelet function due to a genetic defect.
- - Findings revealed that adolescents/adults with 22q11.2DS had significantly lower platelet counts and GPIb expression compared to children and healthy controls, with a noted decrease in platelet counts as age increased.
- - Despite these differences, bleeding symptoms were mostly minor and did not vary significantly between children and adolescents/adults, although some patients experienced major bleeding events, highlighting the need for more research on bleeding during surgery and trauma.