Visualization of Nucleic Acids in Microand Nanometer-Scale Biological Objects Using Analytical Electron Microscopy.

Analytical electron microscopy techniques, including energy-dispersive X-ray spectroscopy (EDX) and electron energy-loss spectroscopy (EELS), are employed in materials science and biology to visualize and chemically map diverse elements. This review presents cases of successful identification of nucleic acids in cells and in DNA- and RNA-containing viruses that use the chemical element phosphorus as a marker.

The Dps Protein Protects DNA in the Form of the Trimer.

The Dps protein is the major DNA-binding protein of prokaryotes, which protects DNA during starvation by forming a crystalline complex. The structure of such an intracellular DNA-Dps complex is still unknown. However, the phenomenon of a decrease in the size of the Dps protein from 90 Å to 69-75 Å during the formation of a complex with DNA has been repeatedly observed, and no explanation has been given.

Sxt1, Isolated from a Therapeutic Phage Cocktail, Is a Broader Host Range Relative of the Phage T3.

Using BW25113 as a host, we isolated a novel lytic phage from the commercial poly-specific therapeutic phage cocktail Sextaphage (Microgen, Russia). We provide genetic and phenotypic characterization of the phage and describe its host range on the ECOR collection of reference strains. The phage, hereafter named Sxt1, is a close relative of classical coliphage T3 and belongs to the genus, yet its internal virion proteins, forming an ejectosome, differ from those of T3.

[Analysis of defects in health care delivery for children based on the data of commission forensic medical examinations performed in the Republic of Uzbekistan].

Objective: To perform a retrospective analysis of the commission forensic medical examinations' data of pediatric profile with identification of defects in health care delivery and establishment of their connection with unfavorable outcome.

Material And Methods: The data of 3854 commission forensic medical examinations performed in the period from 2012 to 2021 regarding the evaluation of quality of health care delivery for children with the establishment of nature and type of defects in health care delivery, determination of disease (injury) outcome, identification of cause-effect relationship between the defect in health care delivery and negative disease (injury) outcome.

Results And Conclusions: Defects in health care delivery were equal 9% in the studied commission forensic medical examinations.