[Influence of chemically different antiviral substances on the expression of IFNα, PKR, OAS1a and RNAse L genes].

The aim of the study was to determine the ability of several chemically different antiviral substances to induce the expression of interferon α(IFNα), PKR, OAS1a and RNAse L genes in the rat liver. The investigated substances included Amizon, Altabor and Proteflasid, which are already used in practical medicine, and 3',7-dimethylquercetin extracted from Proteflasid, the mixture of synthesized trimethyl- and tetramethylquercetin and Sialospecific lectin from persimmon, which are at the stage of preclinical trial and experimental research respectively. The content of corresponding mRNAs in total RNA was detected with the help of reverse transcription and polymerase chain reaction in real time.

[Initial analysis of the results of the genome-wide search for the genes responsive to interferon alpha].

The paper is focused on the primary analysis of the potential genes of primary response to IFNalpha, predicted by the program COTRASIF elaborated in our laboratory. Two web-instruments FatiGO and GOTM were applied for this purpose; the literature search was carried out using IHOP; genes were classified according to the conceptual diagram "Gene Ontology" (GO) and were ranked according to their first priority for the experimental validation. On the basis of conducted analysis 162 genes of potential primary response to IFNalpha were subdivided into two groups--61 genes, for which the experimental data of their responsibility to IFNalpha were found and 101 genes for which such data are absent.