[Effect of antioxidant Mannich base acetate on the growth, cyclic AMP level, and Ca(2+) uptake by yeast cells in Saccharomyces cerevisiae].

We have studied the effect of Mannich base acetate (MBA), an antioxidant, on growth of the yeast, as well as on the level of cAMP and Ca2+ uptake by them. We demonstrate that the antioxidant induced an increase in the level of cAMP in yeast cells and a decrease in Ca2+ uptake by the cells. The effect of the antioxidant depends on the level of Ca2+ in the growth medium.

[Effect of exogenous nucleodepolymerases on Bacillus subtilis multiplication].

The effect of exogenous nucleodepolymerases on cell growth was studied with Bacillus subtilis. Nucleodepolymerases of microbial and animal origin stimulated growth of the culture. The stimulating action of DNAases depended on the dose of the enzyme added to the growth medium and on the phase of the cultural growth.

[Effect of pancreatic DNAse on DNA synthesis in Bacillus subtilis].

An addition of pancreatic DNAse to the cultural medium is found to stimulate DNA synthesis and proliferation of Bacillus subtilis cells. Pancreatic DNAse induces a single-stranded disruption of Bac. subtilis DNA, which may act as a mechanism of DNA synthesis increase and of the culture growth acceleration.