Functionalization of MWCNTs for Bioelectrocatalysis by Bacterial Two-Domain Laccase from .

This study was carried out in order to assess several modifications of carbon nanotube-based nanomaterials for their applications in laccase electrodes and model biofuel cells. The modified MWCNTs served as adapters for the immobilization of laccase from VKM Ac-875 on the surface of electrodes made of graphite rods and graphite paste. The electrochemical properties of the electrodes were tested in linear and cyclic voltammetrical measurements for the determination of the redox potential of the enzyme and achievable current densities.

Polymer-Based Conductive Nanocomposites for the Development of Bioanodes Using Membrane-Bound Enzyme Systems of Bacteria in Biofuel Cells.

The development of biofuel cells (BFCs) currently has high potential since these devices can be used as alternative energy sources. This work studies promising materials for biomaterial immobilization in bioelectrochemical devices based on a comparative analysis of the energy characteristics (generated potential, internal resistance, power) of biofuel cells. Bioanodes are formed by the immobilization of membrane-bound enzyme systems of VKM V-1280 bacteria containing pyrroloquinolinquinone-dependent dehydrogenases into hydrogels of polymer-based composites with carbon nanotubes.

Biocompatible Silica-Polyethylene Glycol-Based Composites for Immobilization of Microbial Cells by Sol-Gel Synthesis.

Biocatalysts based on the methylotrophic yeast VKM Y-2559 immobilized in polymer-based nanocomposites for the treatment of methanol-containing wastewater were developed. The organosilica composites with different matrix-to-filler ratios derived from TEOS/MTES in the presence of PEG (SP-composite) and from silicon-polyethylene glycol (STP-composite) differ in the structure of the silicate phase and its distribution in the composite matrix. Methods of fluorescent and scanning microscopy first confirmed the formation of an organosilica shell around living yeast cells during sol-gel bio-STP-composite synthesis.

Isolation and Characterization of Homologically Expressed Methanol Dehydrogenase from AM1 for the Development of Bioelectrocatalytical Systems.

(Ca)-dependent pyrroloquinolinequinone (PQQ)-dependent methanol dehydrogenase (MDH) (EC: 1.1.2.

Impact of hydrophilic polymers in organosilica matrices on structure, stability, and biocatalytic activity of immobilized methylotrophic yeast used as biofilter bed.

The impact of hydrophilic polymers in an organosilica matrix on the features and performance of immobilized methylotrophic yeast cells used as biocatalysts was investigated and described. Yeast cells were immobilized in a matrix made of tetraethoxysilane (TEOS) and methyltriethoxysilane (MTES) by one-step sol-gel route of synthesis in the presence of polyethylene glycol (PEG) or polyvinyl alcohol (PVA). Organosilica shells were spontaneously built around cells as a result of yeast immobilization at a TEOS to MTES ratio of 85/15 vol% and hydrophilic polymer (PEG or PVA).