[Immunity to poliovirus after vaccination of children with type 1 diabetes mellitus and rheumatic diseases].

Aim: To assess immunization coverage against poliomyelitis and level of immunity postvaccination in children with type 1 diabetes mellitus (DM1) and rheumatic diseases.

Materials And Methods: Vaccination status of 299 children with DM1 and 136 children with rheumatic diseases was determined. Serologic test using neutralization reaction was performed in 31 children with DM1 and 29 children with rheumatic diseases.

[Aseptic meningitis season caused by enteroviruses in 2007: molecular-epidemiological aspects].

Aim: Analysis of manifestation of epidemic process of aseptic meningitis and causes of its activation using molecular genetic methods.

Materials And Methods: Samples of feces and CSF, nasopharyngeal swabs from 296 patients with aseptic meningitis (AM), as well as 240 samples of drinking water and 6 samples of lake water were studied. Epidemiologic analysis, isolation of enteroviruses in Hep-2 and RD cell cultures, RT-PCR, partial sequencing of 5'NTR and genome region coding VP1 were performed.

[Postvaccinal immunity to polio-viruses of children with chronic renal pathology].

41 children with chronic renal diseases (with different forms of chronic glomerulonephritis or chronic renal failure) previously vaccinated with 5 to 9 doses of oral poliovaccine (OPV) were examined. Analysis of immunity to polioviruses revealed insufficient serologic protection against all three types of polioviruses compared with general population. Algorithm of yearly serologic examination as well as a schedule of additional vaccination against poliomyelitis depending on chronic renal pathology were developed.

[Optimization of RT-PCR for the identification of VP4 gene of group A rotaviruses and evaluation of its diagnostic efficiency].

A method is suggested for the detection of the human rotavirus VP4 gene by RT-PCR. The method is universal for all [P] types of group A rotavirus in clinical samples. As against RNA electrophoresis and ELISA, it provides for a more accurate detection of rotaviruses by 18.

[Assessment of a developed "nested" variant of polymerase chain reaction for detecting enteroviruses in patients].

The RT-PCR assay for the detection of all human enteroviruses was used to analyze fecal samples, cerebrospinal fluid (CSF), respiratory tract swabs, and sera from 204 patients with symptoms of viral infection. Enteroviruses were detected in 31.37% of cases.