A human placental polydeoxyribonucleotide (PDRN) may promote the growth of human corneal fibroblasts and iris pigment epithelial cells in primary culture.

The optimal concentration of a human placental polydeoxyribonucleotide (PDRN) preparation (100 microg/ml) enhances the growth of human corneal fibroblasts in primary culture depending upon the donor age. In particular, this effect is very consistently reproducible with donors over 60 years of age (p = 0.0028), suggesting a selective benefit of PDRN in the tissue culture of senescent cells.

Antiviral activity of FA-11H on the infectivity and replication of herpes simplex virus type 1 in cultured cells.

The highest concentration lacking cytotoxicity (h.c.l.

Evaluation of the trophic effect of human placental polydeoxyribonucleotide on human knee skin fibroblasts in primary culture.

A simple assay capable of evaluating the trophic effect of growth factors or active principles on human skin diploid fibroblasts in primary culture has been developed. The results indicate that at physiological concentrations (20-100 micrograms/ml) a human placental polydeoxyribonucleotide (PDRN) preparation enhances the growth of human skin diploid fibroblasts of the knee in primary culture. This effect is consistently reproducible in the case of patients over 60 years of age, and may explain previously reported data on the successful clinical applications of human placental preparations, suggesting a selective benefit of PDRN in wound healing when compared to other treatments.

A short-term plaque assay for antiviral drug research on herpes simplex virus type 2.

In antiviral drug research, gelatinized growth medium cannot be used after infection to avoid the development of secondary plaques, because agar or other chemicals may interfere with the assessment of cytotoxic activity of antiviral compounds. In this study we evaluated quantitatively the different expression of cytopathic effects of Herpes Simplex Virus type 2 (HSV-2) in Vero cell cultures utilizing different periods of incubation after infection, and analysed the incidence of viral progeny particles on the formation of satellite plaques in order to define a short-term plaque assay utilizing a single cycle of infection. The results shown in the work suggest using a period of 24 hours of incubation post adsorption for a screening on inhibition of HSV-2 infectivity in antiviral drug studies.

Antiviral activity of S-7HNa on the infectivity and replication of herpes simplex virus type 1 in cultured cells.

The effect of sodium salt of the acid related to ester S-7 (i.e. S-7HNa) on the infectivity and replication of herpes simplex virus type 1 in Vero cultured cells was studied.