Control of ribosome synthesis in Escherichia coli: analysis of an energy source shift-down.

The rate of ribosome synthesis and accumulation in Escherichia coli during the transition after an energy source shift-down was analyzed. The shift was imposed on cultures of stringent and relaxed strains growing in glucose minimal medium by the addition of the glucose analogue alpha-methylglucoside. In the stringent strain, ribosome synthesis was almost instantaneously reduced after the shift, whereas the relaxed strain exhibited a more gradual response.

Control of protein synthesis in Escherichia coli: analysis of an energy source shift-down.

The energy source shift-down described in the preceding paper (Molin et al., J. Bacteriol.