A spectrophotometric and polarographic investigation of three new cyclohexene-substituted benzodiazepines.

Three recently introduced benzodiazepine derivatives, tetrazepam (I), nortetrazepam (II) and menitrazepam (III) have been subjected to spectral and polarographic investigation. From the ultraviolet spectral data their pK(a)-values have been determined: 4.28 (I), 4.

Differential pulse polarography of some benzodiazepines and their determination in urine.

A differential pulse polarographic method has been applied to the determination of diazepam, oxazepam, nitrazepam and flurazepam down to 2 x 10(-7)M (0.14 mug/ml). The method can also be used with biological materials such as urine, without prior extraction.