[Effect of hydrogen sulfide on isolated rat heart reaction under volume load and ischemia-reperfusion].

The present study was aimed to investigate the effect of H2S donor (NaHS) on heart function in conditions of functional loads and ischemia-reperfusion (I/R) injury by using Langendorf isolated heart perfusion. NaHS ("Sigma", 7,4 mg per kg) was dissolved in physiological solution and injected intraperitoneally 30 min before experiment. Rat isolated hearts were Langendorf-perfused and subjected to 20-minute non-flow ischemia followed by 40-minute reperfusion.