[Optimization of cultivation conditions of Cryptococcus albidus--producers of alpha-L-rhamnosidase].

The influence of content of cultivation media and fermentation conditions on synthesis of alpha-L-rhamnosidase by Cryptococcus albidus 1001 and 1003 were studied. It was shown that L-rhamnose and NaNO, are the optimum sources of carbon and nitrogen, respectively. The temperature of 25 degrees C, pH 6.

[Screening of yeast-producers of alpha-L-rhamnosidase].

Screening of producers of alpha-L-rhamnosidase among 331 yeast strains (representatives of 12 genera and 17 species) has been performed. A capacity to synthesize the enzyme was revealed in 45% of the studied strains with activity 0.01-0.

[Investigations of functional groups of Penicillium commune 266 alpha-L-rhamnosidases].

On the basis of the data of metal ions influence it was established that Penicillium commune 266 alpha-L-rhamnosidases are dependent of metals. The cations of Cu2+, Mn2+, Ag+, Hg2+ essentially influenced the activity decreasing its up to 45-55%. Ba2+ and Mn2+ ions completely inhibited alpha-L-rhamnosidase 2 activity in concentration of 10(-3) M.

[Induction of synthesis and activation of Penicillium commune alpha-L-rhamnosidase].

For the first time the influence of a number of synthetic porfirin and fluoren derivatives, coordinative germanium substances and surfactants on the biosynthesis and activity of Penicillium commune 266 alpha-L-rhamnosidase has been studied. It is shown that some of porfirin derivatives and coordinative germanium substances may be used as inducers of biosynthesis (143-430%) and also as stimulators of alpha-L-rhamnosidase activity (15-44%). It is more expedient to use biscitratgermanium acid in a complex with asparaginic acid (430%) or threonine (370%) as inducers of biosynthesis of enzyme.

[Optimization of cultivation conditions of Penicillium palitans strain 266 which synthesizes alpha-L-rhamnosidase].

The influence of different factors on the biosynthesis of extracellular alpha-L-rhamnosidase in Penicillium palitans at submerged cultivation has been investigated. It was shown that rhamnose and NaNO3 are the optimum carbon and nitrogen sources, respectively. The temperature of 26 degrees C, pH 5.