[Are the properties of peripheral blood lymphocytes of patients with prostate cancer connected with the efficiency of radiation therapy?].

The genome damage (frequency of cells with micronuclei and chromosome aberrations), concentration of reactive oxygen forms (ROS), markers of lymphocytes activation, expression of proliferation (CD69, Ki67) and proapoptotic antigen (CD95), as well as the ability to adaptive response have been investigated in blood lymphocytes of healthy donors and patients with prostate gland cancer. The influence of hormone-therapy on lymphocytes properties and connection between the parameters studied with the effectiveness of treatment, which was estimated by the level of prostate specific antigen (PSA), have been investigated. It was discovered that the genome damage to the patients with prostate gland cancer lymphocytes does not differ from control.

[Individual variability of immunological markers, radiosensitivity and oxidative status in blood lymphocytes of Moscow residents].

Expression of activation (CD69) and proliferation (Ki67) markers, their connection with each other, with the oxidative status (reactive oxygen species--ROS) and with radiosensitivity (determined by micronucleus test) have been studied on stimulated blood lymphocytes from Moscow inhabitants. It was shown that the content of T-lymphocytes with the expressed CD69 and the content of T-lymphocytes with the expressed Ki67 markers correlate (r = 0.571; p = 0.

[Cytogenetic and immunological characteristics of stimulated human peripheral blood lymphocytes are connected with cell proliferation rates].

In the current study the authors investigated the connection between the proliferation rate of stimulated hu- man peripheral blood lymphocytes with some other characteristics of the population: expression of immunological markers, spontaneous genome damage, radiosensitivity. The population's proliferation index (PI) was taken as a measure of the rate. It was calculated using the composition of a cell population, which was cyto- kinesis-blocked with a cytochalasin B.

[Changes in cellular radiosensitivity after low dose irradiation].

When the adaptive response (AR) was studied on human blood lymphocytes, a new dependence was discovered. This dependence defines the direction of the radiosensitivity change after a low dose of irradiation. Using micronucleus (MN) test with cytochalasin B the dependence between the cell reaction after low level irradiation and radiosensititvity (the effect after irradiation at the dose of 1 Gy) was observed.

[The disturbances of the relationship between oxidative homeostasis and the immune status of lymphocytes in liquidators of the consequences of the Chernobyl accident].

We investigated into the relations between the immune status of individuals who took part in liquidating the consequences of the Chernobyl accident (liquidators) and the level of active oxygen forms in peripheral blood lymphocytes, as well as the level of the genome damage in lymphocytes (frequency of cells with micronuclei). The results show that the immune status changes as the level of the genome damage increases: the content of some markers increases and others decreases. It has also been shown that a) active oxygen forms participate in forming some indexes of the immunological lymphocyte status and b) the exposure of liquidators to irradiation many years ago almost completely changes the characteristics of the relation between the concentration of active oxygen forms and immunological status indexes.