Publications by authors named "O M Alessa"
Article Synopsis
- The study presents a new phagemid-based system that produces CRISPR-Cas13a-loaded antibacterial capsids (AB-capsids) specifically targeting multidrug-resistant Staphylococcus aureus.
- By optimizing phagemid copy numbers, researchers achieved higher yields and purity of AB-capsids, illustrating a direct relationship between phagemid quantity and capsid production.
- The developed AB-capsids effectively eliminate targeted S. aureus strains while leaving non-target strains unharmed, showcasing their potential as effective tools against antibiotic-resistant bacteria.
Article Synopsis
- The lab created a phagemid system to generate CRISPR-Cas13a-antimicrobial capsids specifically targeting MRSA to combat rising antimicrobial resistance.
- They faced a challenge with unwanted wild-type phage production during packaging, which was addressed by introducing silent mutations to reduce contamination while maintaining efficiency.
- The optimized system showed effective sequence-specific killing of MRSA strains but highlights the need for further research on its effectiveness against other bacteria and in real body conditions.
Article Synopsis
- The study focuses on oxacillin-susceptible methicillin-resistant Staphylococcus aureus (OS-MRSA), which poses new treatment challenges due to its ability to develop resistance through chromosomal mutations.
- Researchers analyzed six mutant strains with decreased oxacillin susceptibility, examining how mutations in RNA polymerase (RNAP) genes led to transcription dysfunction and an accumulation of certain metabolites.
- The findings indicated that these mutations resulted in cell wall thickening, which ultimately decreased the mutants' susceptibility to β-lactam antibiotics, highlighting the need for a better understanding of these mechanisms for effective clinical management.
BACKGROUND Familial hypomagnesemia with secondary hypocalcemia (HSH) is a rare autosomal recessive disorder (OMIM# 602014) caused by mutations in the gene encoding transient receptor potential melastatin 6 (TRPM6)) on chromosome 9q22, a channel involved in epithelial magnesium resorption. While a plethora of studies have delineated various clinical manifestations pertinent to this mutation, the literature is devoid of connections between TRPM6 mutations and bleeding diathesis, or sudden infant death syndrome (SIDS). This report presents a case of familial HSH associated with the novel homozygous TRPM6 gene variant c.
View Article and Find Full Text PDFBackground: Monitoring protocols have been developed because patients taking atypical antipsychotics are more prone to developing metabolic syndrome, which leads to possible increased mortality and morbidity. The aim of this study was to assess the degree of adherence to the recommendations of metabolic syndrome monitoring.
Materials And Methods: This study was conducted in two large psychiatric facilities in the Eastern Province of Saudi Arabia.