Publications by authors named "O Landt"
Article Synopsis
- Febrile illnesses are a major health issue in sub-Saharan Africa, often leading to inadequate treatment due to limited diagnostic resources and a wide range of possible pathogens.
- This study focused on evaluating a multiplex polymerase chain reaction (PCR) method for diagnosing febrile diseases in patients at a hospital in Central Ethiopia over two years, testing samples from 511 patients.
- The results showed that while pathogens were detected in only 6.3% of the samples, including Plasmodium and Borrelia, the use of multiplex PCR can still enhance diagnostic capabilities and inform better clinical management in settings with limited resources.
Background: Many newborn screening programs worldwide have introduced screening for diseases using DNA extracted from dried blood spots (DBS). In Germany, DNA-based assays are currently used to screen for severe combined immunodeficiency (SCID), spinal muscular atrophy (SMA), and sickle cell disease (SCD).
Methods: This study analysed the impact of pre-analytic DNA carry-over in sample preparation on the outcome of DNA-based newborn screening for SCID and SMA and compared the efficacy of rapid extraction versus automated protocols.
Article Synopsis
- The study evaluates the effectiveness of different antibody tests for diagnosing Ascaris lumbricoides infections in school children, comparing these with standard methods like copromicroscopy and quantitative PCR (qPCR).
- The research found that copromicroscopy methods had relatively low sensitivity, while qPCR proved to be more reliable in detecting infections.
- Specifically, the IgG1 antibody response against adult Ascaris excretory-secretory products emerged as a highly accurate diagnostic tool, indicating its potential to enhance existing diagnostic techniques for monitoring Ascaris infections.
Objective: Genomic surveillance and seroprevalence of severe acute respiratory syndrome coronavirus-2 (SARS CoV-2) in Bangladesh is paramount for COVID-19 pandemic preparedness yet lagging the high-income countries due to limited resources.
Methods: SARS-CoV-2 variants, COVID-19 symptoms, and serology were prospectively evaluated in a cross-sectional study of Bangladeshi adults testing RT-PCR positive in 2021 and 2022.
Results: SARS CoV-2 Omicron variants of asymptomatic or mild COVID-19 in 2022 replaced Delta variant infections requiring hospitalization and oxygen support.
This study was performed to comparably assess two commercial real-time PCR assays for the identification of DNA in serum. A total of 518 Colombian serum samples with high pre-test probability for infections with either or apathogenic were assessed. The assessment comprised the NDO real-time PCR (TIB MOLBIOL, ref.
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