Rice anther culture: callus initiation and androclonal variation in progenies of regenerated plants.

Anthers from rice (Oryza sativa L.) subspecies japonica initiated more callus than their indica or indica x japonica counterparts. A mild stress, either by slow desiccation or heat shock, prior to the plating of anthers enhanced the ability to initiate callus.

Regeneration from long-term cell suspension cultures of tepary bean (Phaseolus acutifolius).

Plant regeneration has been achieved from long-term cell suspension cultures established from leaf derived callus of tepary bean (Phaseolus acutifolius). The proportion of densely cytoplasmic cells in suspension culture increased when cultured in the L-6 medium with 54 μM NAA and 2 μM KN. Filtration of the cells at each of five consecutive subcultures resulted in the isolation of a plant regenerating cell line (TB 686), which is being maintained in L-6 medium with 4.

Plant regeneration from cell suspension cultures of Vigna aconitifolia.

Plant regeneration has been achieved routinely from established cell suspension culture lines of Vigna aconitifolia (moth bean), a highly drought tolerant grain legume. The cultures originated from three-week-old leaf callus. Several media including MS, B5, AA, SL, PCM, SH and L-6 were tested for their effects on cell growth.

Cell division and differentiation in protoplasts from cell cultures of Glycine species and leaf tissue of soybean.

Protoplasts were isolated from cell cultures of G. soja and G. tabacina, respectively.