Publications by authors named "O Krut"
Since therapeutic options are limited the utilization of prebiotics is suggested to prevent food allergies (FAs). Using an experimental peach allergy model we explored the effect of dietary fiber pectin, a high-methoxyl heteropolysaccharide, on the manifestation of FA. CBA/J mice were sensitized, subsequently orally boosted and provoked with peach peel extract.
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- Clostridioides difficile (C. difficile) is a major cause of healthcare-related diarrhea, with issues like antibiotic resistance and high relapse rates complicating treatment.
- *Faecal microbiota transplantation is a potential therapy but understanding the key factors for successful colonization resistance is necessary for its broader application.
- *Experts highlighted the need for a Controlled Human Infection Model (CHIM) to safely study mild to moderate C. difficile infections, which could lead to new treatments and better insights into how the infection works.*
Article Synopsis
- The study focuses on improving blood safety measures by validating Transfusion-Relevant Bacterial Reference Strains (TRBRS) in a lyophilised format, making them easier to use compared to current methods requiring ultra-cold storage.
- Two bacterial strains, Klebsiella pneumoniae and Staphylococcus aureus, were lyophilised and sent to 11 labs globally to test their effectiveness in identifying and monitoring growth in platelet concentrates.
- Results showed that the lyophilised TRBRS maintained growth properties and stability, allowing for straightforward addition to platelet concentrates, and demonstrated effectiveness across all participating labs, indicating potential for broader application in blood safety quality control.
Within the Innovative Health Initiative (IHI) Inno4Vac CHIMICHURRI project, a regulatory workshop was organised on the development and manufacture of challenge agent strains for Controlled Human Infection Model (CHIM) studies. Developers are often uncertain about which GMP requirements or regulatory guidelines apply but should be guided by the 2022 technical white paper "Considerations on the Principles of Development and Manufacturing Qualities of Challenge Agents for Use in Human Infection Models" (published by hVIVO, Wellcome Trust, HIC-Vac consortium members). Where those recommendations cannot be met, regulators advise following the "Principles of GMP" until definitive guidelines are available.
View Article and Find Full Text PDFDysregulated B cell responses have been described in inflammatory bowel disease (IBD) patients; however, the role of B cells in IBD pathology remained incompletely understood. We here provide evidence for the detrimental role of activated B cells during the onset of autoimmune intestinal inflammation. Using Wiskott-Aldrich Syndrome interacting protein deficient (Wipf1) mice as a mouse model of chronic colitis, we identified clusters of differentiation (CD)86 expression on activated B cells as a crucial factor exacerbating pro-inflammatory cytokine production of intestinal CD4 T cells.
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