Experimental Method for the Measurements and Numerical Investigations of Force Generated on the Rotating Cylinder under Water Flow.

The paper presents the experimental test setup and measurement method of hydrodynamic force generated on the rotating cylinder (rotor) under uniform flow including the free surface effect. The experimental test setup was a unique construction installed in the flume tank equipped with advanced flow generating and measuring systems. The test setup consisted of a bearing mounted platform with rotor drive and sensors measuring the hydrodynamic force.

Hanoverian F/W-line contributes to segregation of Warmblood fragile foal syndrome type 1 variant PLOD1:c.2032G>A in Warmblood horses.

Background: Warmblood fragile foal syndrome (WFFS) is a lethal condition detected in Warmblood horses. Its origin and association with performance traits and fertility among horse populations is unknown.

Objectives: To validate the previously identified WFFS type 1 (WFFST1)-associated missense variant PLOD1:c.

Biocatalytic response of multi-layer assembled collagen/hyaluronic acid nanoengineered capsules.

Biodegradable hollow capsules filled with fluorescently labelled bovine serum albumin (BSA) as a model drug were prepared via layer-by-layer (LbL) self-assembly of type-I collagen (COL) and hyaluronic acid (HA) using calcium carbonate micro-particles and co-precipitation method. Capsules loaded with fluorescein isothiocyanate (FITC)-BSA, tetramethylrhodamin isothiocyanate (TRITC)-BSA or Alex-Fluor-488-BSA, respectively, were characterised before and after core removal using Confocal Laser Scanning Microscopy (CLSM), whilst the morphologies of individual hollow capsules were assessed using Atomic Force Microscopy (AFM). The sustained release of the encapsulated FITC-BSA protein was attained using enzymatic degradation of the capsule shells by collagenase.

Multicompartmental micro- and nanocapsules: hierarchy and applications in biosciences.

Multicompartmentalized micro- and nanocapsules allow simultaneous delivery of several vectors or biomolecules; they are the next generation of carriers with increased complexity. Here we overview multicompartment micro- and nanocapsules and present a road-map for future developments in the field. Four basic building block structures are demonstrated, three isotropic: concentric, pericentric, and innercentric, and one anisotropic: acentric.

Controlled intracellular release of peptides from microcapsules enhances antigen presentation on MHC class I molecules.

To understand the time course of action of any small molecule inside a single cell, one would deposit a defined amount inside the cell and initiate its activity at a defined moment. An elegant way to achieve this is to encapsulate the molecule in a micrometer-sized reservoir, introduce it into a cell, remotely open its wall by a laser pulse, and then follow the biological response by microscopy. The validity of this approach is validated here using microcapsules with defined walls that are doped with metallic nanoparticles so as to enable them to be opened with an infrared laser.