[Cytological characteristics of carminomycin-resistant P-388 lymphoid leukosis].

The carminomycin-resistant strain (P-388/c) of P-388 lymphoid leukemia is obtained. Resistance development is accompanied by higher sensitivity to cytostatics, rise of hyperploidy, replacement of the stem line, presence of definite markers in leukemic cells as well as by the appearance of a clone of cells with higher dry mass. At the level of CFUs after the carminomycin administration more remote terms of cell restoration than in case of P-388/c lymphoid leukemia are determined.

[Hemopoietic precursor cells during the development of carminomycin resistance].

A comparative quantitative investigation of colony-forming ability of mouse bone marrow hemopoietic stem cells in the case of ordinary leukemia variant (P-388) and carminomycin-resistant variant (P-388/k) was carried out. Leukemia bearing mice received single injections of increasing doses of carminomycin on the 5th day after transplantation and 24 hours later the bone marrow was investigated for quantity of colony-forming units in the spleen (CFUs). Dynamics investigation of bone marrow cells in the case of P-388 and P-388/k (without treatment) reveals the common tendency to a decrease of these cells in the period before animal death.

[Colony-forming ability of bone marrow hematopoietic cells in mice with transplanted leukemia during administration of carminomycin].

Effects of carminomycin on the colony-forming ability of bone marrow haemopoietic stem cells (CFUs) were compared in normal mice with steady state and actively regenerating bone marrow, and in P-388 and La leukemia-bearing mice. CFUs assays were performed 24 h after treatment of donor mice with the increasing doses of the drug. Leukemia-bearing mice received carminomycin on the 5th day after transplantation.

[Antileukemic activity of carminomycin complexes with a high-polymer DNA].

Complexes of carminomycin with DNA were prepared from lyophilized carminomycin and high polymer DNA of sturgeon milt. The complex formation was evident from a batochromic shift in the maxima at 450-600 nm and an increase in the absorption level at 545 nm. The carminomycin complexes with DNA had a relatively low general toxicity and higher antileukemic activity in studies on mice with tumor L-1210 as compared to the use of carminomycin alone.