Exploring multidisciplinary team members' experiences of supporting eating and drinking with acknowledged risk in healthcare and aged care settings.

Purpose: Dysphagia is a swallowing condition which has significant health and quality of life implications. Speech-language pathologists provide assessment and support shared decision making to optimise swallowing safety. Many people elect to eat and drink with acknowledged risk.

Fluorinated -Heterocyclic Carbene Silver(I) Complexes with High Cancer Cell Selectivity.

This work presents the synthesis of five new functionalized (benz)imidazolium -heterocyclic (NHC) ligands () and four new (benz)imidazole silver(I) NHC (Ag(I)-NHC) complexes of mononuclear or binuclear type. The complexes have been fully characterized, including single crystal X-ray diffraction of three new structures. The complexes and their corresponding free NHC ligands have been screened against breast cancer and noncancerous cell lines, showing the mononuclear benzimidazole complex has the highest activity, while the binuclear benzimidazole complex has the highest cancer cell selectivity.

CHiMP: deep-learning tools trained on protein crystallization micrographs to enable automation of experiments.

A group of three deep-learning tools, referred to collectively as CHiMP (Crystal Hits in My Plate), were created for analysis of micrographs of protein crystallization experiments at the Diamond Light Source (DLS) synchrotron, UK. The first tool, a classification network, assigns images into categories relating to experimental outcomes. The other two tools are networks that perform both object detection and instance segmentation, resulting in masks of individual crystals in the first case and masks of crystallization droplets in addition to crystals in the second case, allowing the positions and sizes of these entities to be recorded.

A self-complementary AAV proviral plasmid that reduces cross-packaging and ITR promoter activity in AAV vector preparations.

Adeno-associated viral vectors (AAVs) are a leading delivery system for gene therapy in animal models and humans. With several Food and Drug Administration-approved AAV gene therapies on the market, issues related to vector manufacturing have become increasingly important. In this study, we focused on potentially toxic DNA contaminants that can arise from AAV proviral plasmids, the raw materials required for manufacturing recombinant AAV in eukaryotic cells.