Modeling the behavior of monoclonal antibodies on hydrophobic interaction chromatography resins.

Monoclonal antibodies (mAbs) require a high level of purity for regulatory approval and safe administration. High-molecular weight (HMW) species are a common impurity associated with mAb therapies. Hydrophobic interaction chromatography (HIC) resins are often used to remove these HMW impurities.

GFP reporter screens for the engineering of amino acid degrading enzymes from libraries expressed in bacteria.

There is significant interest in engineering human amino acid degrading enzymes as non-immunogenic chemotherapeutic agents. We describe a high-throughput fluorescence activated cell sorting (FACS) assay for detecting the catalytic activity of amino acid degrading enzymes in bacteria, at the single cell level. This assay relies on coupling the synthesis of the GFP reporter to the catalytic activity of the desired amino acid degrading enzyme in an appropriate E.

De novo engineering of a human cystathionine-γ-lyase for systemic (L)-Methionine depletion cancer therapy.

It has been known for nearly a half century that human tumors, including those derived from the nervous system such as glioblastomas, medulloblastoma, and neuroblastomas are much more sensitive than normal tissues to l-methionine (l-Met) starvation. More recently, systemic l-Met depletion by administration of Pseudomonas putida methionine-γ-lyase (MGL) could effectively inhibit human tumors xenografted in mice. However, bacterial-derived MGLs are unstable in serum (t(1/2) = 1.

Is third party information true? Should blood donors be excluded because of it?

The Welsh Blood Service contacted and attempted to interview 40 donors about whom information from a third party had been received. This information would have excluded the donor from donation if it had been forthcoming from the donor. 57.