One-step cloning and targeted duplication of Pantoea ananatis chromosomal fragments.

In this study, we describe a novel method for one-step cloning and targeted duplication of P. ananatis chromosomal fragments. According to this method, the chromosomal region of interest is subcloned in vivo via λ Red recombination into the short synthetic non-replicable DNA fragment containing the excisable antibiotic-resistance marker gene and φ80 att-P site.

A novel one-step method for targeted multiplication of DNA fragments from the Escherichia coli chromosome mediated by coordinated functioning of λ and φ80 bacteriophage recombination systems.

A novel technique for targeted stable multiplication of a specific long E. coli chromosome fragment was developed. The method is based on the coordinated functioning of λ and φ80 bacteriophage site-specific recombination and integration systems.