Structural insights into Cullin4-RING ubiquitin ligase remodelling by Vpr from simian immunodeficiency viruses.

Viruses have evolved means to manipulate the host's ubiquitin-proteasome system, in order to down-regulate antiviral host factors. The Vpx/Vpr family of lentiviral accessory proteins usurp the substrate receptor DCAF1 of host Cullin4-RING ligases (CRL4), a family of modular ubiquitin ligases involved in DNA replication, DNA repair and cell cycle regulation. CRL4DCAF1 specificity modulation by Vpx and Vpr from certain simian immunodeficiency viruses (SIV) leads to recruitment, poly-ubiquitylation and subsequent proteasomal degradation of the host restriction factor SAMHD1, resulting in enhanced virus replication in differentiated cells.

[Treatment of patients wtih malignant lymphoma with freeze-dried cord blood].

It has been ascertained that in patients with malignant lymphomas, employment of cryopreserved cells of the cord blood (CB) is associated with a high efficiency of therapy of the underlying medical condition. With introduction of CB into the test-system of the donor's blood the T-lymphocytes radiosensitivity has been shown to be increased during the Go- and S-periods of the cell cycle, which fact should be taken account of in planning radiotherapy in oncological patients. A decrease in the level of aneuploid lymphocytes against the background of blast-transformation as a result of transfusion of CB may suggest normalization of the pool of lymphocytes.