Network pharmacology and molecular docking-based identification of drug candidates and key targets of for colorectal cancer treatment.

Colorectal cancer (CRC) is a type of cancer with high morbidity and mortality in several developing and developed countries of the world. Its mortality and morbidity are predicted to increase over the next decade, hence, efforts aimed at combating it have remained unabated. In the context of its treatment, the use of chemotherapeutics is often limited by challenges including cost-ineffectiveness, side effects, and drug resistance.

Identification of a 1, 8-naphthyridine-containing compound endowed with the inhibition of p53-MDM2/X interaction signaling: a computational perspective.

Various studies have established that molecules specific for MDMX inhibition or optimized for dual inhibition of p53-MDM2/MDMX interaction signaling are more suitable for activating the Tp53 gene in tumor cells. Nevertheless, there are sparse numbers of approved molecules to treat the health consequences brought by the lost p53 functions in tumor cells. Consequently, this study explored the potential of a small molecule ligand containing 1, 8-naphthyridine scaffold to act as a dual inhibitor of p53-MDM2/X interactions using computational methods.

Comparative study of the reproductive toxicity and modulation of enzyme activities by crude oil-contaminated soil before and after bioremediation.

Contamination of soil with crude oil is a serious ecological problem with potential adverse public health effects. This study assessed the germ cell toxicity of simulated leachates from crude oil-contaminated soil before and after bioremediation using the murine sperm abnormality assay, sperm count, and testes histopathology. The levels of Total Testosterone (TT), Follicle Stimulating Hormone (FSH), and Luteinizing Hormone (LH); and activities of catalase (CAT), alkaline phosphatase (ALP), superoxide dismutase (SOD), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were determined.

Levels of polybrominated diphenyl ethers (PBDEs) in water and sediment from open city drains in Makurdi Metropolitan Area, North Central Nigeria.

Polybrominated diphenyl ethers (PBDEs) have been classified as persistent organic pollutants (POPs) as well as endocrine-disrupting compounds (EDCs). This study determined the concentrations of six PBDE congeners (BDE 47, BDE 99, BDE 100, BDE 153, BDE 154, and BDE 183) in water and sediment samples from open city drains, in the Makurdi Metropolitan Area, North Central Nigeria, using gas chromatogragh-mass spectrometer. These congeners are components of the penta- and octa-BDE formulations that have been banned by the European Union.

In silico design and validation of a highly degenerate primer pair: a systematic approach.

Background: The techniques of amplifying genetic materials have enabled the extensive study of several biological activities outside the biological milieu of living systems. More recently, this approach has been extended to amplify population of genes, from evolutionarily related gene family for detection and evaluation of microbial consortial with several unique potentialities (e.g.