[The use of DNA probes for the diagnosis of pulmonary tuberculosis].

Mycobacterium-specific nonradioactive DNA probes have been tested able to detect mycobacteria in clinical samples (sputum, bronchoalveolar lavage) for 48 hours. The probes sensitivity makes up 1000-10,000 cells. Molecular hybridization confirms the results of DNA-probe diagnostic conclusion of tuberculosis more frequently than the results of standard microbiological tests.

[The identification of alphaviruses by using cDNA probes complementary to the 3'-terminal of the viral genome].

Venezuelan equine encephalomyelitis, Eastern equine encephalomyelitis and Sindbis viruses, as well as members of various serological groups of alphaviruses were identified using synthesized molecular probes complementary to 3'-terminus of the molecule of the appropriate genomic RNA: 32P-single-stranded kDNA and E. coli-cloned 32P-double-stranded kDNA. A high species-specificity of kDNA-probes was demonstrated.