Endocannabinoid system upregulates the enrichment and differentiation of human iPSC- derived spermatogonial stem cells via CB2R agonism.

Background: Male factor infertility (MFI) is responsible for 50% of infertility cases and in 15% of the cases sperm is absent due to germ cell aplasia. Human induced pluripotent stem cell (hiPSC)-derived spermatogonial stem cells (hSSCs) could serve as an autologous germ cell source for MFI in patients with an insufficient sperm yield for assisted reproductive technology (ART). The endocannabinoid system (ECS) has been implicated to play a role in mouse embryonic stem cells (mESCs) and the human testicular environment.

Basil chilling injury: Oxidative stress or energy depletion?

Basil (Ocimum basilicum L.) is susceptible to chilling injury (CI), leading to significant postharvest quality loss. This research aimed to identify key metabolites involved in CI of basil during cold storage to better understand the underlying mechanisms.

Electrorheological Fluids Based on Porous Carboxyl-Functionalized Polytriphenylamines.

Electrorheological fluids (ER) make up a class of smart materials that are distinguished by their capacity to alter their rheological characteristics in a controlled and reversible manner in response to an externally applied electric field (). As an inherent polarizable anhydrous ER-active material, polyaniline (PAni)-based materials are among the most frequently utilized ER-active materials. However, PAni can only be used as an ER-active material after carefully adjusting its conductivity to an appropriate range.

Identification of Flavonoids Using UV-Vis and MS Spectra.

Flavonoids are a group of specialized metabolites that are ubiquitously found within the plant kingdom. While they fulfill various important functions within the plant, they are also utilized by humans in a variety of different fields such as medicine, food science, and agriculture. Thus, to elucidate the chemical composition of any given plant extract, extraction and identification of flavonoids are of high interest.

Correction of Griscelli Syndrome Type 2 causing mutations in the gene with CRISPR/Cas9.

Background/aim: Griscelli Syndrome Type 2 (GS-2) is a rare, inherited immune deficiency caused by a mutation in the gene. The current treatment consists of hematopoietic stem cell transplantation, but a lack of suitable donors warrants the development of alternative treatment strategies, including gene therapy. The development of mutation-specific clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 gene editing technology has opened the way for custom-designed gene correction of patient-derived stem cells.