[Experimental and clinical studies of drug regulation of the anti-lysozyme activity of microorganisms causing opportunistic infections].

Experimental materials on choosing antibiotics for etiotropic therapy of opportunistic infections with an account of the regulating effect of the drugs on the ++anti-lysozyme activity of pathogens (the factor of intracellular parasitism) are presented. The in vitro data were applied to the clinical trials in 30 patients with chronic and acute pyelonephritis of the Proteus etiology and to 25 patients with chronic inflammatory diseases of Staphylococcus etiology. It was shown that the use of the antibiotics which lowered the ++anti-lysozyme activity of microorganisms promoted a more rapid disappearance of the disease clinical signs, increased 2- to 3-fold the terms of the remission and resulted in an increase in the number of the persons with complete remission (54.

Cholesterol can stimulate secretion of apolipoprotein B by cultured human hepatocytes.

During a 5 day cultivation of human hepatocytes in a primary culture the secretion of apolipoprotein B was measured by enzyme-linked immunosorbent assay. Density-gradient ultracentrifugation demonstrated that the majority of the secreted apolipoprotein B was associated with the very-low-density lipoprotein fraction. Exposure of the cells to cholesterol (5-100 micrograms/ml) resulted in a dose-dependent increase in apolipoprotein B secretion rate.

Heterogeneity of endothelium in human aorta. A quantitative analysis by scanning electron microscopy.

Scanning electron microscopy was used to characterize a silver-stained endothelial surface of human aorta and iliac artery. The autopsy material was taken 1-3 h after death from two age groups: group A (0-7 yr) and group B (40-75 yr). The mean cell density per 1 mm2 in the arteries of group A was significantly higher than in the arteries of group B which lie outside ostia, fatty streaks and plaques.

[Scanning microscopy of hamster cells transformed by herpes simplex virus type 2].

Scanning microscopy was used to examine the features of morphology and attachment to a solid substrate of a transformed and tumor lines of hamster cells. These cell lines differed from normal hamster fibroblasts by changes in the mode of attachment and the degree of flattening on the solid substrate, relief of the cell surface and pattern of intercellular interactions. The observed morphological changes correlated with the degree of cell transformation.