Publications by authors named "O D Stringer"
The COVID-19 pandemic has highlighted the need for rapid and reliable diagnostics that are accessible in resource-limited settings. To address this pressing issue, we have developed a rapid, portable, and electricity-free method for extracting nucleic acids from respiratory swabs (i.e.
View Article and Find Full Text PDFArticle Synopsis
- Recombinase polymerase amplification (RPA) is a technique used to detect infectious agents by amplifying nucleic acids, but it relies heavily on the match between primers and templates in the reaction.* -
- Mismatches in this primer-template complex can slow down the reaction, affect the accuracy of results, and lead to false negatives, yet their effects in RPA are less understood compared to other methods like PCR.* -
- The study revealed that specific mismatches, especially in the 3'-anchor region of the primer, significantly impact RPA performance, with some combinations completely stopping the reaction, thereby helping researchers optimize RPA for better diagnostic use.*
(APP) is the causative agent of porcine pleuropneumonia, resulting in high economic impact worldwide. There are currently 19 known serovars of APP, with different ones being predominant in specific geographic regions. Outbreaks of pleuropneumonia, characterized by sudden respiratory difficulties and high mortality, can occur when infected pigs are brought into naïve herds, or by those carrying different serovars.
View Article and Find Full Text PDFThe Gram-negative bacterium is the causative agent of pleuropneumonia in pigs, its only known natural host. Typical symptoms of peracute disease include fever, apathy and anorexia, and time from infection to death may only be 6 h. Severe lung lesions result from presence of one or two of the ApxI-III toxins.
View Article and Find Full Text PDF