Matrix metalloproteinases correlate with alveolar-capillary permeability alteration in lung ischemia-reperfusion injury.

Background: Matrix metalloproteinases (MMPs) are able to degrade the endothelial basal lamina and increase vascular permeability.

Methods: In a porcine model of isolated-reperfused lung, we studied the alveolar-capillary permeability and the zymographic expression of MMP-9 and MMP-2 in the bronchoalveolar lavage fluid of lungs submitted ex vivo to ischemia in three preservation solutions [modified Euro-Collins (EC), low-potassium-dextran, modified-blood]. Twenty-two pigs were randomly divided into three groups according to the preservation solution used.

Beneficial effects of leukocyte-depleted blood and low-potassium dextran solutions on microvascular permeability in preserved porcine lung.

Modified Euro-Collins (EC) solution, a crystalloid intracellular-type solution, has been commonly used for pulmonary preservation. Several experimental studies have shown the advantages of using extracellular colloid-based solutions. The aim of this study was to compare the quality of preservation of two extracellular colloid solutions, leukocyte-depleted blood (BL) and low-potassium dextran (LPD) solutions, with that of EC solution.

[Preoperative evaluation of hemorrhagic risk].

Should we consider that haemostasis tests may help guide the anaesthesiologist's therapeutic choices when the patients is on the operating table and bleeds without evident explanation? The response is yes, provided that the results will be available rapidly. Haemostasis tests that are performed in laboratory wards distant from the surgery room often prove to be unuseful in emergency situations as haemostasis abnormalities usually occur in a shorter time than is required to get biological test results. Devices located in the vicinity of the surgery room should therefore be available to the anaesthesiologist.