EFFICIENCY OF PHOTON CAPTURE BEAM TECHNOLOGY AND PHOTODYNAMIC IMPACT ON MALIGNANT AND NORMAL HUMAN CELLS IN VITRO.

Objective: to investigate the structural and morphofunctional changes in test systems of malignant (cell lineA549) and normal (stem fibroblasts) human cells exposed to X-rays in the presence of gadoliniumcontaining photon capture agent «Dotavist» and optical light (red spectrum) in combination with «Fotolon» photosensitizer.

Methods: The continuous cell culture of normal human fibroblasts and malignant human cells technology, X-ray and red light exposure, cytological and statistical methods.

Results: Effects of the two binary radiation technologies, namely the photon capture impact on malignant cells(human nonsmall cell lung cancer cells i.

SELECTION AND TESTING OF EXPERIMENTAL MODELS OF NORMAL AND MALIGNANT HUMAN CELLS IN VITRO AND EVALUATION OF THEIR SENSITIVITY RANGE TO THE NEUTRON/CAPTURE AND PHOTON/CAPTURE AGENTS AND PHOTOSENSITIZERS.

Objective: to investigate the structural and morpho-functional changes in test systems of malignant (A-549 cellline) and normal (fibroblasts of the 6th passage) human cells during incubation with gadolinium-containing pho-ton-capture agent «Dotavist» and photosensitizer «Fotolon».

Methods: The passaged (continuously interweaved) cell culture technique on normal human fibroblasts and malig-nant human cells; cytological, biophysical, statistical methods.

Results: The cytotoxic properties of «Dotavist» gadolinium-containing photon-capturing agent and «Photolon»photosensitizer in a wide range of concentrations (5, 10, 25, 50, 100 and 200 μl/ml) were studied by the morpho-functional characteristics (growth kinetics, proliferative and mitotic activity, presence of atypical cells) in the invitro test systems of malignant (non-small cell lung cancer cell line A-549) and normal (6th passage fibroblasts)human cells.