[Determination, at equilibrium, of association constants of labelled or unlabelled ligands by a non-graphical method (author's transl)].

The present work deals with the determination of association constants at equilibrium by a non-graphical method in binding systems containing one specific receptor. Equations have have been derived from that originally described by Lea (Biochim. Biophys.

[Method of rapid separation of free, sulfoconjugated and glucuronoconjugated fractions of plasma steroids].

A method for the separation of serum conjugated steroids, sulfates and glucuronides, is described. It is based on the fact that methylene blue forms complexes with ester-sulfates which can be quantitatively and specifically extracted by chloroform from aqueous phase. The quantitative determination of the conjugated fractions thus obtained can be done after solvolysis followed by hydrolysis by beta-glucuronidase from Helix pomatia.