Autoradiographically detected impairment of RNA synthesis pattern in 8-16 cell bovine embryos after irradiation.

Early preimplantation bovine embryos at 8- or 16-cell stage were analysed by [5-3H]uridine autoradiography for distribution of newly synthesized RNA after 60Co irradiation with a single dose of 1 Gy, 2 Gy or 4 Gy gamma rays, respectively. Embryos irradiated with a single dose of 1 Gy showed equally decreased synthesis of RNA in nucleoplasma as well as in nucleolus. In embryos irradiated with a single dose of 2 Gy or 4 Gy, RNA synthesis was decreased and localized mostly on the periphery of the nucleus; in both cases of irradiation, the nucleus center being without labelling.

Effect of K, MG aspartate on some biological parameters of aging rats.

The effect of K and Mg salts of aspartic acid (Cardilan) on the serum concentration of selected proteins and phagocytic activity in aging male Wistar rats was investigated. Cardilan was administered in tap water for 7 days a month for 3 months before the last observed interval (12, 18 and 24 month). In a part of animals, the aging process was accelerated by sublethally gamma-irradiation.

Detection of genome impairment in bovine early embryos by autoradiography: a methodological note.

The applicability of Pavlok's method characterising the nuclear status of early preimplantation bovine embryos by nuclear labelling pattern after a short pulse of [5-3H]uridine (revealing in situ detection of RNA transcription at the onset of the major embryonic transcription) was tested on experimentally irradiated 8- to 16-cell bovine embryos. After [5-3H]uridine labelling the semi-thin sections of these embryos were analysed by autoradiography for intranuclear distribution of newly synthesised RNA expected to be influenced by increasing doses of irradiation by gamma rays from a 60Co source. In control embryos, the labelling was homogeneously distributed in nucleoplasm and in nucleoli.

Influence of age and K, Mg aspartate (Cardilan) on murine haemopoiesis.

We have studied the effect of age as well as the effect of short-term and long-term intake of K and Mg salts of aspartic acid (Cardilan) on haemopoiesis in ICR mice strain. The cellularity of the bone marrow does not change with aging, but the number of granulocyte-macrophage colony-forming cells (GM-CFC) and also the number of spleen colony-forming units (CFU-S) and erythroid burst-forming units (BFU-E) in two-year-old mice increased in the bone marrow. In two-year-old mice the number of leukocytes decreased in the peripheral blood with aging, mainly as a result of a decrease in mononuclear cells.

Favourable effect of K, Mg aspartate on serum proteins in aging rats.

The serum concentration of individual proteins in aging rats was studied. We have made an attempt to slow down the changes in concentration occurring in rats from 5 up to 17 months of age by application of aspartate given in drinking water containing 500 mg% of monopotassium DL asparagicum and 500 mg% of monomagnesium D,L asparagicum. The solution was administered continuously for 14 days a month for 1 year.