Seabirds as anthropization indicators in two different tropical biotopes: A One Health approach to the issue of antimicrobial resistance genes pollution in oceanic islands.

Antimicrobial resistance is a quintessential One Health issue, among the most serious 21st century global threats to human health. Seabirds may act as sentinels of natural and anthropogenic changes in the marine ecosystem health, including pollution by antimicrobial resistance genes (ARGs). We used real time PCR to identify and quantify 22 plasmid-mediated ARGs in the gastrointestinal microbiome of six wild seabird species, comparing an anthropized (Fernando de Noronha Archipelago - FNA) and a pristine biotope (Rocas Atoll - ROA), Brazil.

Carnivore Parvovirus Ecology in the Serengeti Ecosystem: Vaccine Strains Circulating and New Host Species Identified.

Carnivore parvoviruses infect wild and domestic carnivores, and cross-species transmission is believed to occur. However, viral dynamics are not well understood, nor are the consequences for wild carnivore populations of the introduction of new strains into wild ecosystems. To clarify the ecology of these viruses in a multihost system such as the Serengeti ecosystem and identify potential threats for wildlife conservation, we analyzed, through real-time PCR, 152 samples belonging to 14 wild carnivore species and 62 samples from healthy domestic dogs.

Transmission ecology of canine parvovirus in a multi-host, multi-pathogen system.

Understanding multi-host pathogen maintenance and transmission dynamics is critical for disease control. However, transmission dynamics remain enigmatic largely because they are difficult to observe directly, particularly in wildlife. Here, we investigate the transmission dynamics of canine parvovirus (CPV) using state-space modelling of 20 years of CPV serology data from domestic dogs and African lions in the Serengeti ecosystem.

[Agreement in the determination of electrolytes in samples of serum and plasma. Stability of these samples kept for 2 hours at room temperature with uncovered tubes].

Unlabelled: The determination of electrolytes in the clinical laboratories is carried out in samples of serum or plasma in situations that tubes remain open during a lingering time.

Objective: To estimate the agreement and the interchangeability in the determination of electrolytes for three types of tubes (serum with separator, plasma with and without separator) in samples that remain at room temperature and open up to two hours.

Material And Methods: Samples of 36 voluntary patients were selected determining the levels of sodium, potassium, chlorine, calcium, magnesium and phosphate in the three tubes on time 0 (basal), 60 and 120 minutes.