Pangenomic insights into evolution and acquisition of functional genes for bioremediation.

is a genus of organohalide-respiring bacteria that is recognized for its fastidious growth using reductive dehalogenases (RDases). In the SC05 culture, however, a population also mineralizes dichloromethane (DCM) produced by chloroform dechlorination using the cassette, just downstream of its active RDase. A closed genome of this DCM-mineralizing lineage has previously evaded assembly.

Two distinct metagenome-assembled genomes from anaerobic chloroform and dichloromethane degrading consortia.

Here we present two metagenomes and two metagenome-assembled genomes from subcultures of an anaerobic chloroform and dichloromethane degrading microbial community used for bioremediation. Our objective was to assemble and curate the genome(s) of , key biodegraders in the culture, through repeated sequencing and joint assembly with previous datasets.

Metagenome from ACT-3/CF: an anaerobic chloroform-degrading microbial community.

Here, we present the metagenome of an anaerobic chloroform respiring mixed microbial community used for bioremediation. Our objective was to obtain draft genomes of key microorganisms in the culture.

Vitamin B as a source of variability in isotope effects for chloroform biotransformation by Dehalobacter.

Carbon and chlorine isotope effects for biotransformation of chloroform by different microbes show significant variability. Reductive dehalogenases (RDase) enzymes contain different cobamides, affecting substrate preferences, growth yields, and dechlorination rates and extent. We investigate the role of cobamide type on carbon and chlorine isotopic signals observed during reductive dechlorination of chloroform by the RDase CfrA.

Metagenomic sequences from anaerobic chloroform and dichloromethane degrading microbial communities.

Here, we present metagenomes from two cultures derived from an anaerobic microbial consortium used for bioremediation. One culture dechlorinates chloroform to dichloromethane, which is further mineralized to CO. A second subculture was amended with only dichloromethane.