Publications by authors named "O Bogado Pascottini"
Previous studies in the horse highlight the potential benefit of prolonged in vitro maturation (IVM) (34 h) compared to short IVM (24 h) with or without prior oocyte holding, but little is known about the optimal IVM duration within this interval. To determine the effect of oocyte holding and duration of IVM ranged between 28 and 34 h on nuclear maturation, cleavage, blastocyst formation, and pregnancy rates, a retrospective study was performed in an equine clinical OPU-ICSI setting. The study included data of 2114 aspirated oocytes from 201 OPU-ICSI sessions.
View Article and Find Full Text PDFFollowing a significant increase in herd and farm sizes after the removal of milk quotas in Europe, the past 10 years have seen a slight yet steady decline in the population of cattle. This includes a reduction of approximately 5 % in dairy and beef cattle. This trend is driven by various factors, such as changing market demands, economic shifts, and sustainability challenges in the livestock sector.
View Article and Find Full Text PDFEffect of type of anticoagulant, transportation time, and glucose in the culture media on neutrophil viability and function test results in dairy cattle.
PLoS One
October 2024
Article Synopsis
- The study investigates how various factors affect the immune function of dairy cattle's white blood cells (PMN), especially their ability to engulf pathogens (phagocytosis) and produce reactive oxygen species (oxidative burst) using blood samples from Holstein heifers.
- Blood samples were collected in tubes with different anticoagulants (ACD vs. EDTA) and stored at a cool temperature for varying lengths of time to analyze PMN viability and functionality.
- Results showed that while PMN viability dropped significantly after 6 hours of storage for both anticoagulant types, there was no notable difference in PMN function (phagocytosis and oxidative burst) between the two anticoagulants or across the different storage times. *
Background: Within the follicular fluid, extracellular vesicles (EVs) guide oocyte growth through their cargo microRNAs (miRNAs). Here, we investigated the role of EVs and their cargo miRNAs by linking the miRNAs found in EVs, derived from the fluid of an individual follicle, to the ability of its oocyte to become a blastocyst (competent) or not (non-competent).
Methods: Bovine antral follicles were dissected, categorized as small (2-4 mm) or large (5-8 mm) and the corresponding oocytes were subjected to individual maturation, fertilization and embryo culture to the blastocyst stage.
High levels of reactive oxygen species (ROS) derived from in vitro conditions compromise oocyte quality and subsequent polyspermy prevention by the zona and membrane block. Antioxidant supplementation, like lycopene, during in vitro maturation (IVM) mitigates ROS effects, yet, its efficacy in blocking polyspermy remains uncertain. This study aims to evaluate the effect of lycopene supplementation during IVM on oocyte maturation, fertilization, and developmental parameters.
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