[Influence of Ca2+ ions on metabolism of active oxygen species in combined cultures of wheat calluses with the fungus Tilletia caries].

The effect of Ca2+ on morphophysiological parameters of calluses of wheat Triticum aestovum L., the level of active oxygen species, and the activity of oxalate oxidase, peroxidase, and catalase is investigated in the case of infestation with the fungus Triticum aestivum causing ball smut. The concentration of O2-, H2O2, and activity of oxidoreductases (oxalate oxidase, peroxidase, and catalase) depends on the content of Ca2+ ions in the culture medium of calluses.

[Plant resistance inductors and active forms of oxygen. III. The influence of bisol 2 and baitan on morphogenesis and defense response of wheat non-morphogenic callus cells infected with smut agent].

A study was made of the influence of bisol 2 and baitan compounds on morphogenesis and defence response of wheat calluse cells infected with bunt agent in associated with oxalate oxidase activation. After introduction of bisol and baitan into cultivation medium, dense area with meristema zones, germs of shoots and rhizoids appeared on non-morphogenic calluses, which correlated with enzyme activation. Parenchyma-like cells, generating hydrogen peroxide, were seen in the site of pathogen penetration under infestation, but were never revealed in control.

[Plant resistance inductors and active forms of oxygen. II. The influence of chitooligosaccharides on the production of hydrogen peroxide with the involvement of oxalate oxidase in common cultures of wheat calluses and bunt pathogen].

We have studied the influence of various concentrations of chitooligosaccharides (CO) on hydrogen peroxide (H2O2) generation, with the involvement of oxalate oxidase (OO), in rhizoids and in zones of hard brand pathogen penetration, as well as on oxalate oxidase activity in wheat calluses. In the control group, diaminobenzidin (DAB)-staining was typical of 30% of peripheral rhizoid cells, which provided their resistance during infection. In the zone of fungus penetration, the appearance of DAB-stained parenchyma-like cells was observed.

[Plant resistance inductors and active forms of oxygen. I. The influence of salicylic acid on hydrogen peroxide production in common cultures of wheat callus and bunt pathogen].

The influence of salicylic acid (SA) on cell resistance to bunt pathogen in wheat calluses has been studied. Cell staining by diaminobenzidin substratum (DAB-cells) was conditioned by hydrogen peroxide (H2O2) generation with the involvement of oxalate oxidase (OO). In the control group, DAB-staining was typical only of rhizoid cells (up to 50%).

[Development of blunt Tilletia caries (D.C.) Tul. pathogen on embryogenic wheat callus].

The development of Tilletia caries on embryogenic and morphogenic wheat calluses from germination of spores up to sorus formation have been investigated. Pathogene growth was similar in variants with wheat calluses of susceptible species Triticum aestivum and resistant species T. timopheevii, but intensity of its development of embryogenic calluses of both species was lower, than on morphogenic.