Impact of N-Terminal PEGylation on Synthesis and Purification of Peptide-Based Cancer Epitopes for Pancreatic Ductal Adenocarcinoma (PDAC).

Peptide-based cancer vaccines have shown promising results in preclinical trials focusing on tumor immunotherapy. However, the presence of hydrophobic amino acid segments within these peptide sequences poses challenges in their synthesis, purification, and solubility, thereby hindering their potential use as cancer vaccines. In this study, we successfully synthesized peptide sequences derived from mesothelin (MSLN), a tumor-associated antigen overexpressed in pancreatic ductal adenocarcinoma (PDAC) by conjugating them with monodisperse polyethylene glycol (PEG).

Inflammatory Biomarkers and Gait Impairment in Older Adults: A Systematic Review.

Peripheral inflammation and gait speed alterations are common in several neurological disorders and in the aging process, but the association between the two is not well established. The aim of this systematic literary review is to determine whether proinflammatory markers are a positive predictor for gait impairments and their complications, such as falls in older adults, and may represent a risk factor for slow gait speed and its complications. The systematic review was performed in line with the Preferred Report Items for Systematic Review and Meta-Analyses (PRISMA).

A frontal air intake may improve the natural ventilation in urban buses.

In this report we analyze the air flow across the open windows (natural ventilation) of an urban bus model and the consequent dispersion of aerosols emitted in the passengers area. The methods include computational fluid dynamics simulations and three ways to characterize the dispersion of passive tracers: a continuous concentration-based model, a discrete random model and a parametric scalar based on the so-called mean age of air. We also conducted experiments using a 1:10 scale bus model and [Formula: see text] as a passive tracer to assess the ventilation characteristics.

Improving Juvenile Justice Settings by Decreasing Coercion: One Lab's Perspectives from Behind the Fence.

In this article, we outline an emerging role for applied behavior analysis in juvenile justice by summarizing recent publications from our lab and discussing our procedures through the lens of coercion proposed by Goltz (2020). In particular, we focus on individual and group interventions that target a range of behaviors emitted by adolescents in a residential treatment facility. In general, individual interventions involve teaching adolescents to (1) respond appropriately to staff, (2) tolerate nonpreferred environmental conditions, and (3) control problematic sexual arousal.

Tea Bags for Fmoc Solid-Phase Peptide Synthesis: An Example of Circular Economy.

Peptide synthesis is an area with a wide field of application, from biomedicine to nanotechnology, that offers the option of simultaneously synthesizing a large number of sequences for the purpose of preliminary screening, which is a powerful tool. Nevertheless, standard protocols generate large volumes of solvent waste. Here, we present a protocol for the multiple Fmoc solid-phase peptide synthesis in tea bags, where reagent recycling steps are included.